The pure cultures of actinomycetes isolates under study were
inoculated in starch casein broth at 32 C for one week. After incubation,
the broth was centrifuged at 8,000 g min1 for 10 min at
10 C and supernatant was discarded. The pellet was washed with
sterile distilledwater (SDW) twice and re-suspendedin SDW to reach
a cfu 106 ml1 approximately (called bioagent suspension hereafter).
R. solani (Accession No. NAIMCC F-01971) was obtained from the
National Agriculturally Important Microbial Culture Collection,
India and used as a test pathogen. The pathogenic fungus was disk
inoculated in wheat bran (700 g in 2 l capacity flask; moisture level
50%) and incubated for two weeks in the dark with proper mixing
at regular intervals for uniform distribution of fungal mycelia.