For determination of SOD and GPx activity and TAA and MDA concentration, erythrocytes
were and mixed with cold redistilled water and
stand at +4 °C for 15 minutes to lyses and then stored in 1.5-mL microtubes at −80°C. Hemoglobin (Hb) concentration was analyzed colorimetrically with a ZiestChem Diagnostic
kit (catalog no. 10-532, Ziest Chemistry, Tehran, Iran) for determination of SOD and GPx
activity (U/g Hb) and TAA and MDA concentration.