Raman spectroscopy is a label free technique that identifies different
constituents of the biological sample based on its molecular
vibration. The beauty of Raman spectroscopy lies in its specificity
where each biomolecule give rise to its own characteristic spectral
peak distinguish them from other molecules. In case of overlap
of Raman peaks of different components, one can distinguished
them in term of principle components such as Protein, lipids and
nucleotides [11,12].
The Raman spectra contain strong fluorescence background
because of the presence of natural fluorophores present in the biological
samples. In addition, it also contains noise that might add
from detector electronics. Therefore all the Raman spectra from
healthy as well as diseased samples have been processed in Matlab
environmentfor the removal of noise and fluorescence background
[13]. Fig. 1 part (a) shows the fluorescence background subtracted
normalized Raman spectra of blood sera of 25 normal healthy
samples and part (b) shows the normalized Raman spectra of 40
confirmed dengue virus infected sera. The spectra of normal as well
dengue infected samples have been smoothed by using average
filter, Savitzky–Golay, with five points and 3rd order polynomial