Soil, in particular, is an extensively explored ecological niche for microorganisms that produce useful biologically active natural products including antibiotics [34]. Majority of antibiotics so far isolated were produced by Streptomycetes and fungi, which are common inhabitants of the soil [15]. Modern researchers emphasize on the need to explore novel unexplored niches like rain forests [28] marine sponges [12] mangroves [16] and endophytes [25,29] for pharmacologically active compounds. The expanding list of novel microorganisms and the products derived from poorly explored areas of the world like Australia [19], Jordan [21] and Antarctica [18] suggests that a careful exploration of new habitats might continue to be useful. The biopharmaceutical industry has become increasingly interested in novel antibiotics to meet the challenge of resistance. By employing strategic screening programmes of microbes, it is feasible to increase the number of potential products for therapeutic use [13,27]. In the present paper, we present the report on fungal isolates showing antimicrobial activity from the hitherto unexplored soil and dung samples collected from the Kaziranga National Park, declared as one of the World Heritage site by UNESCO in 1985. The park is a unique natural landscape of sheer forest, tall elephant grass, rugged reeds, marshes and shallow pools [31].