Previously, there was no report on the spectrophotometric determination of FA in biological
sample. The method being followed in the present investigation could be a good alternative to
conventional methodological approach such as HPLC for concentration measurement of FA.
Literature on fermentation-based production of FA shows that the presence of (only qualitative)
fumaric acid was determined by the formation of insoluble mercurous fumarate in 5 %
nitric acid as no method for exact quantification of FA was available [20]. This might have
urged many researchers to develop a reliable colorimetric method for the determination of FA
concentration in biological samples. However, probably pertaining to the rapid development in
the modernization and sophistication of analytical instruments such as HPLC, this method of
FA estimation was not explored anymore. The present investigation has highlighted and
improvised a method important in both economical and analytical prospective.