The cells were washed once more with 1mL of deionizedwater, and the pelletwas suspended in 1 mL of TES buffer (25 mM sucrose, 50 mM Tris HCl, pH 8.0, 10mMEDTA) with 10 mg/mL of lysozyme before incubating for 1 h at 37 C.
The cells were washed once more with 1mL of deionizedwater, and the pelletwas suspended in 1 mL of TES buffer (25 mM sucrose, 50 mM Tris HCl, pH 8.0, 10mMEDTA) with 10 mg/mL of lysozyme before incubating for 1 h at 37 C.