indicated that the Salmonella shell contamination at the
end of storage was strongly correlated with the trans-shell penetration
as was found in this study. The 2% chitosan coating significantly
decreased trans-shell penetration. The slightly lower numbers of
Salmonella on shells of agar-filled eggs coated with the 2% chitosan at
the end of storage compared to the numbers on uncoated eggs, could
have caused the lower trans-shell penetration. On the other hand, the
Salmonella shell contamination, determined on whole eggs, was not
reduced by the 2% chitosan coating. It can be suggested that the
slightly lower Salmonella shell contamination using agar-filled eggs
with 2% chitosan could have resulted from the presence of an
obstruction against a possible nutrient flow from the agar in the shell
to the bacteria on the shell. If the chitosan coating can form a barrier to
a nutrient flow, it may also form an additional physical barrier against
trans-shell penetration. This is supported by the finding that the
numbers of open pores in eggshells coated with 1% and 2% chitosan
was about four times less than the numbers of open pores in uncoated
eggshells. Nevertheless, the 1% chitosan coating did not reduce transshell
penetration
indicated that the Salmonella shell contamination at theend of storage was strongly correlated with the trans-shell penetrationas was found in this study. The 2% chitosan coating significantlydecreased trans-shell penetration. The slightly lower numbers ofSalmonella on shells of agar-filled eggs coated with the 2% chitosan atthe end of storage compared to the numbers on uncoated eggs, couldhave caused the lower trans-shell penetration. On the other hand, theSalmonella shell contamination, determined on whole eggs, was notreduced by the 2% chitosan coating. It can be suggested that theslightly lower Salmonella shell contamination using agar-filled eggswith 2% chitosan could have resulted from the presence of anobstruction against a possible nutrient flow from the agar in the shellto the bacteria on the shell. If the chitosan coating can form a barrier toa nutrient flow, it may also form an additional physical barrier againsttrans-shell penetration. This is supported by the finding that thenumbers of open pores in eggshells coated with 1% and 2% chitosanwas about four times less than the numbers of open pores in uncoatedeggshells. Nevertheless, the 1% chitosan coating did not reduce transshellpenetration
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