The present authors have previously reported the partial
purification of cell wall glucan by using the NaClO oxidation
method (21). Oxidized A. fumigatus cells were prepared
from the β-glucan (laminarin)-added and control
cultures, and their structure analyzed by 13C-NMR spectroscopy
and enzymolysis using zymolyase, endo β-1,3-
glucanase. The amount of zymolyase-susceptible component
was examined (Table 2). In the sucrose and glucoseadded
cultures, it was lower than the detection limit
(