In this study, we investigated the effect of thermoresponsive poly-
mers, pNIPAM and p(NIPAM-co-AA), on enzymatic cell wall disruption
and lipid recovery from microalga,
Chlorella protothecoides
.Inparticular,
we demonstrated how the properties of the polymer (amine content)
and mixing conditions (polymer loading, mixing time, mixing sequence)
correlate with important algal cellular compound recovery metrics,
including cell disruption, and lipid and sugar recovery. Enhancement in
cell disruption could be attributed to i) electrostatic interactions with
the positively charged polymer and the algal cell wall, which stresses
and disrupts the membrane, and/or ii) reduction of enzyme denaturation
in the presence of the polymer. Importantly, we also showed that the
copolymers cause cell disruption in the absence of enzyme, providing
an interesting low-cost alternative to enzymatic degradation, especially
considering the recyclability of the thermoresponsive polymers
In this study, we investigated the effect of thermoresponsive poly-mers, pNIPAM and p(NIPAM-co-AA), on enzymatic cell wall disruptionand lipid recovery from microalga,Chlorella protothecoides.Inparticular,we demonstrated how the properties of the polymer (amine content)and mixing conditions (polymer loading, mixing time, mixing sequence)correlate with important algal cellular compound recovery metrics,including cell disruption, and lipid and sugar recovery. Enhancement incell disruption could be attributed to i) electrostatic interactions withthe positively charged polymer and the algal cell wall, which stressesand disrupts the membrane, and/or ii) reduction of enzyme denaturationin the presence of the polymer. Importantly, we also showed that thecopolymers cause cell disruption in the absence of enzyme, providingan interesting low-cost alternative to enzymatic degradation, especiallyconsidering the recyclability of the thermoresponsive polymers
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