2.1.3. Commercial sesame quantification kits
For the quantification of sesame protein in the muffin dough
and in the baked muffins, a commercial sandwich ELISA kit (Biokits,
Sesame Assay Kit 902070X) from NEOGEN (Lansing, MI,
USA) was used. The sesame concentration of the sample materials
is calculated using a calibration curve from a protein standard
solution contained in the kit.