by 30 cycles of denaturation (94C for 50 s), annealing
(51C for 1 min), and primer extension (72C for 1 min),
followed by final extension step for 10 min at 72C.
Amplification products were electrophoretically resolved
on 1.4% (w/v) agarose gel containing ethidium bromide,
using 19 TAE buffer at 70 V.