After 14-days of growth at 25 C and 150 rpm/min, half the volume (250 mL) of ethyl acetate (EtOAc) was added into the flask and the mixture was mixed well using a shaker for 10 min. After being allowed to stand for 1 h, the top phase was transferred into a round flask and condensed in a rotatory evaporator at 37 C. Following complete evaporation, the extract was obtained and dissolved in acetone:water (9:1, v/v).