High performance liquid chromatography (HPLC) UV Detector is capable of analyzing a wide range of substances. For accurate results High accuracy. Some herbs can be detected antioxidants (antioxidant) which has the ability to resist or prevent diseases such as cancer, heart disease, diabetes and inflammation. The research aims to determine the amount of antioxidants 2 types of Vitamin C (Ascorbic acid) and vitamin E (alpha -tocopherol) to develop a proper analysis by HPLC before introduced. Vitamin C measured at a wavelength of 250 nm and vitamin E measurements at wavelengths 205 nm.
The choice of mobile phase and the ratio of mobile phase to suit the needs of the measure. The results , limit of detection (LOD) of vitamin C (Ascorbic. acid) was 0.000041 p4pm and R2 was 0.999 . Limit of detection(LOD) of vitamin E (alpha – tocopherol) was 0.000089 ppm and the R2 was 0.997.
six species of specimens that researchers have studied such Lagerstroemia speciosa ,horseradish (Moringaoleifera Lam.), Lotus (Nelumb onucifera Gaetg), Yanang (Tiliacoratriandra (Colebr.)) , Prieng (Derris scandens Benth) and anchor Thailand (Terminaliachebula Retz)) not found vitamin E (alpha - tocopherol) and vitamin C (Ascorbic acid).
High performance liquid chromatography (HPLC) UV Detector is capable of analyzing a wide range of substances. For accurate results High accuracy. Some herbs can be detected antioxidants (antioxidant) which has the ability to resist or prevent diseases such as cancer, heart disease, diabetes and inflammation. The research aims to determine the amount of antioxidants 2 types of Vitamin C (Ascorbic acid) and vitamin E (alpha -tocopherol) to develop a proper analysis by HPLC before introduced. Vitamin C measured at a wavelength of 250 nm and vitamin E measurements at wavelengths 205 nm. The choice of mobile phase and the ratio of mobile phase to suit the needs of the measure. The results , limit of detection (LOD) of vitamin C (Ascorbic. acid) was 0.000041 p4pm and R2 was 0.999 . Limit of detection(LOD) of vitamin E (alpha – tocopherol) was 0.000089 ppm and the R2 was 0.997. six species of specimens that researchers have studied such Lagerstroemia speciosa ,horseradish (Moringaoleifera Lam.), Lotus (Nelumb onucifera Gaetg), Yanang (Tiliacoratriandra (Colebr.)) , Prieng (Derris scandens Benth) and anchor Thailand (Terminaliachebula Retz)) not found vitamin E (alpha - tocopherol) and vitamin C (Ascorbic acid).
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