Particle size distribution measurements of the homogenized biomass were used to determine if significant cell rupture was achieved ( Spiden et al., 2013). Only a slight broadening in the size distribution peak was observed for biomass preconditioned for 5 h, indicating that the cells had not been sufficiently weakened to enable effective cell rupture by high pressure homogenization ( Fig. 1). Following incubation for 15 h and high pressure homogenization, the resulting multimodal size distributions showed a peak at a particle size significantly smaller than the initial primary particle size of approximately 3 μm ( Fig. 1) indicating that near-complete disruption of the cells had occurred ( Spiden et al., 2013). Consistent results were found on tests with all four batches of paste incubated for 15 h prior to high pressure homogenisation at 1200 ± 100 bar, demonstrating the effectiveness of this method on weakening Nannochloropsis sp. cells in concentrated pastes. The exact mechanisms of cell weakening were not investigated in this work; however it is likely the result of autolytic or exogenous enzymatic action. Regardless, incubation was consistently effective across all batches of algae grown at different times and conditions, and the lipid content of the algae was not detrimentally affected.
Particle size distribution measurements of the homogenized biomass were used to determine if significant cell rupture was achieved ( Spiden et al., 2013). Only a slight broadening in the size distribution peak was observed for biomass preconditioned for 5 h, indicating that the cells had not been sufficiently weakened to enable effective cell rupture by high pressure homogenization ( Fig. 1). Following incubation for 15 h and high pressure homogenization, the resulting multimodal size distributions showed a peak at a particle size significantly smaller than the initial primary particle size of approximately 3 μm ( Fig. 1) indicating that near-complete disruption of the cells had occurred ( Spiden et al., 2013). Consistent results were found on tests with all four batches of paste incubated for 15 h prior to high pressure homogenisation at 1200 ± 100 bar, demonstrating the effectiveness of this method on weakening Nannochloropsis sp. cells in concentrated pastes. The exact mechanisms of cell weakening were not investigated in this work; however it is likely the result of autolytic or exogenous enzymatic action. Regardless, incubation was consistently effective across all batches of algae grown at different times and conditions, and the lipid content of the algae was not detrimentally affected.
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