In the present study, 199 bacterial cultures were isolated from petroleum hydrocarbon contaminated soil. Among
them, 14 strains were selected in terms of their emulsification ability. The potent biosurfactant producer
Pseudomonas stutzeri DB1 was selected as it gave maximum biomass and biosurfactant yield. The isolate also
showed strong emulsification ability. Thus the ability of isolate DB1 (Pseudomonas stutzeri DB1) to produce
biosurfactant with potent emulsification properties suggests its potential application in environment clean up
procedures.