Taking together the results of five experiments presented in this paper and three pilot
studies (data not shown), we can conclude that the i.p. application of AZAGLY alone, in
the form of one single injection (40–80 Ag/kg) or two injections (40 + 40 Ag/kg or 5 + 75
Ag/kg), with a 12-h interval is able to provoke ovulation in 40–60% of females. We can
assume that better broodstock and environmental conditions would contribute to better
treatment results. Egg quality after treatment with AZAGLY is comparable with that
obtained by routine (Ovopel) treatment. It should also be stated that, despite a lower
percentage of ovulated fish as well as a longer duration of ovulation in comparison with
Ovopel, the method of inducing ovulation by AZAGLY alone can be considered as a
useful alternative in the European Union and in future European Union countries when the
use of dopamine antagonists will became illegal in aquaculture practice.