The released target then found another capture DNA to trigger another polymerization cycle, which was repeated for many rounds, resulting in the multiplication of the DNA–invertase conjugation on the surface of Streptavidin-MNBs.
The bound DNA–invertase can be used to catalyze the hydrolysis of sucrose into glucose with millions of turnovers, which transformed the concentration of target DNA into the level of glucose for monitoring of PGM.