The biodiesel obtained from WCO was composed of 19.63% of saturated FAs, 36.35% of monounsaturated FAs, and 44.02% of polyunsaturated FAs whereas the biodiesel obtained from fresh soybean oil was composed of 16.82% of saturated FAs, 26.60% of monounsaturated FAs, and 56.58% of polyunsaturated FAs.