Melting point and solid fat content (SFC) of LH butter and the
control were analysed using a differential scanning calorimeter
(DSC 1, Mettler Toledo, Switzerland) according to the method performed
by Vithanage, Grimson, and Smith (2009). The instrument
was calibrated with indium at a scan rate of 10 C/min using an
empty aluminium pan as a reference. Nitrogen gas was used to
prevent condensation in the cell during analysis. Approximately
10 mg of LH butter and control butter were weighed separately
and loaded into 40 ll aluminium pans and sealed hermetically.
An empty pan was used as a reference. Pans were heated at
10 C/min from 20 to 70 C with heat-cool-reheat cycle under
N2 stream. The peak temperature was calculated by STARe Software
(Version 12.0x) (Mettler Toledo, Switzerland) to determine
the peak melting temperature. The SFC values were determined
at any particular temperature by partial integration of the DSC
curve in percentage.