When the trait variation rate of the M2
-generation plants was less than 85% of the
lower limit value or greater than 115% of the upper limit value of the mutagenic parent, Luhua
11 (except for pod shape), we suspected that mutation had occurred in these plants.
The seeds were harvested separately for each plant in 2012 and were planted in 2013
following the same methods used in 2012. The plants growing in the field in 2013 were the
M3
-generation.