In this study, the aquaculture wastewater treatment was performed in 2 L working volume in batch scale with 14 days treatment period. Both ammonia and phosphorus were almost completely removed from the aquaculture wastewater for the first 7 days and the period of 14 days seems to be appropriate for batch
scale experiment. In addition, treatment period lesser than 14 days would omit the re-inoculation of EM which would complicate the treatment performance in batch scale experiment.