Scanning electron microscopy. The washed granules were placed in sealed 50-ml serum bottles which contained 5% glutaraldehyde in anaerobic cacodylate buffer, and fixation was executed overnight at 4°C. To obtain cleaved preparations of the granules, the fixed samples were quick-frozen in liquid nitrogen and cleaved with a mortar and pestle. Whole and cleaved granules were dehydrated through graded series of water-ethanol and ethanol-Freon 113. The samples were placed on aluminum specimen mounts, coated with goldpalladium, and examined with a Hitachi S-450 scanning ,um in diameter). A few chain-forming rods (approximately 0.6 by 1.6 ,um) which exhibited angular shapes similar to those of Methanothrix spp. were occasionally observed on
the surface. A collapsed extracellular polymer was observed to be associated with the bacteria on the surface, particularly with the large coccoid organisms (Fig. 4).
Scanning electron microscopy. The washed granules were placed in sealed 50-ml serum bottles which contained 5% glutaraldehyde in anaerobic cacodylate buffer, and fixation was executed overnight at 4°C. To obtain cleaved preparations of the granules, the fixed samples were quick-frozen in liquid nitrogen and cleaved with a mortar and pestle. Whole and cleaved granules were dehydrated through graded series of water-ethanol and ethanol-Freon 113. The samples were placed on aluminum specimen mounts, coated with goldpalladium, and examined with a Hitachi S-450 scanning ,um in diameter). A few chain-forming rods (approximately 0.6 by 1.6 ,um) which exhibited angular shapes similar to those of Methanothrix spp. were occasionally observed onthe surface. A collapsed extracellular polymer was observed to be associated with the bacteria on the surface, particularly with the large coccoid organisms (Fig. 4).
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