Explants were implanted onto Murashige and Skoog's (MS) medium [15] containing different phytohormones (6-benzylaminopurine (BAP), indole-3-acetic acid (IAA), Kinetin (Kn) and Indole3-butyric acid (IBA) supplemented with 3% sucrose and 0.8% Difco Bacto-agar in test tubes and culture vessels. The pH of the medium was adjusted to 5.8 before autoclaving and the culture condition was maintained at 24±2oC under 12 h cool white fluorescent tube light (4000 lux) [6].