2. Materials and methods
2.1. Experimental design and protocols
The indoor laboratory used in this experiment was
equipped with 52-L, glass aquaria (76 cm×31 cm ×31 cm)
that received flow-through seawater from one of two sources;
clear well water pumped from a seawater aquifer or
pond water pumped from a 337-m2 round pond used for
intensive shrimp culture.Wellwater was the source water for
the round pond, so any differences in water quality between
well and pond water resulted from inputs into and management
strategies affecting the round pond. Previous studies
indicate that water from the round pond can contain high
concentrations of suspended organic matter, including microalgae
and microbial–detrital aggregates, whereas well
water typically has low concentrations of organicmatter and
is devoid of microalgae (Moss and Pruder, 1995; Otoshi
et al., 2001; Divakaran and Moss, 2004).Water in the pond
wasmixed by a 1-HP paddlewheel that operated nightly, and
pond water was transferred into the laboratory by a 1/4-HP
submersible pump placed 0.5 m below the water surface at
the outer perimeter of the pond. The round pond had amean
water depth of 1 m. (For more details about the round pond,
see Wyban and Sweeney, 1989).
Twenty-four 52-L aquaria were stocked with 0.68-g
(SD+0.02 g) Pacific white shrimp, L. vannamei, at a
density of 24 shrimp/aquaria (100 shrimp/m2 equivalent).
Shrimp were produced at the Oceanic Institute
2. Materials and methods
2.1. Experimental design and protocols
The indoor laboratory used in this experiment was
equipped with 52-L, glass aquaria (76 cm×31 cm ×31 cm)
that received flow-through seawater from one of two sources;
clear well water pumped from a seawater aquifer or
pond water pumped from a 337-m2 round pond used for
intensive shrimp culture.Wellwater was the source water for
the round pond, so any differences in water quality between
well and pond water resulted from inputs into and management
strategies affecting the round pond. Previous studies
indicate that water from the round pond can contain high
concentrations of suspended organic matter, including microalgae
and microbial–detrital aggregates, whereas well
water typically has low concentrations of organicmatter and
is devoid of microalgae (Moss and Pruder, 1995; Otoshi
et al., 2001; Divakaran and Moss, 2004).Water in the pond
wasmixed by a 1-HP paddlewheel that operated nightly, and
pond water was transferred into the laboratory by a 1/4-HP
submersible pump placed 0.5 m below the water surface at
the outer perimeter of the pond. The round pond had amean
water depth of 1 m. (For more details about the round pond,
see Wyban and Sweeney, 1989).
Twenty-four 52-L aquaria were stocked with 0.68-g
(SD+0.02 g) Pacific white shrimp, L. vannamei, at a
density of 24 shrimp/aquaria (100 shrimp/m2 equivalent).
Shrimp were produced at the Oceanic Institute
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