Once transferred back into water there was a further increase in oxygen
consumption, which was maintained for the 6 h recovery period. A
logical explanation would be repayment of an oxygen debt. However,
lactic acid was not detected in any samples, suggesting no anaerobic
metabolism was taking place, and while the PO2 of the coelomic fluid
decreased in 25 °C and 15 °C, it initially increased in 5 °C air. In addition,
the PO2 of the coelomic fluid was rapidly regained when the starfish
were transferred back to water. The increased oxygen consumption
may be associated with increased ionoregulatory requirements to reestablish
intraorganismal ion levels to replace of the fluid lost during
emersion. Blue mussels also show an increase in oxygen consumption
upon return to seawater, it is suggested that this is a general stress
response associated with up-regulation of genes involved in protein rescue
and cellular repair (Place et al., 2011). Although it is possible that
the starfish may have been affected by handling stress when transferred
between the experimental chambers, the fact that oxygen uptake either
dropped or remained unchanged when they were initially transferred
into air does not fully support this idea.
Once transferred back into water there was a further increase in oxygenconsumption, which was maintained for the 6 h recovery period. Alogical explanation would be repayment of an oxygen debt. However,lactic acid was not detected in any samples, suggesting no anaerobicmetabolism was taking place, and while the PO2 of the coelomic fluiddecreased in 25 °C and 15 °C, it initially increased in 5 °C air. In addition,the PO2 of the coelomic fluid was rapidly regained when the starfishwere transferred back to water. The increased oxygen consumptionmay be associated with increased ionoregulatory requirements to reestablishintraorganismal ion levels to replace of the fluid lost duringemersion. Blue mussels also show an increase in oxygen consumptionupon return to seawater, it is suggested that this is a general stressresponse associated with up-regulation of genes involved in protein rescueand cellular repair (Place et al., 2011). Although it is possible thatthe starfish may have been affected by handling stress when transferredbetween the experimental chambers, the fact that oxygen uptake eitherdropped or remained unchanged when they were initially transferredinto air does not fully support this idea.
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