Chlorella sp. IM-01 is cultured in BG-11 media with initial OD 0.01 at 2000 lux light intensity and 27 ± 3°C temperature for 25 days to observe the growth pattern and optical density is read at 680 nm (Fig. 1). This figure shows that initially cells are stabilizing themselves in the medium upto 2nd day after that the growth rate increases upto 6th day. From 6th to 17th day growth rate is maximum. Hence, microalgae utilize nutrients as a very fast rate in this period. Afterwards, growth of microalgae stops or somewhat decreasing.