Solid–liquid extraction
Extraction from blackcurrant bud at the boiling point of
each solvent (hexane, a-pinene, MeTHF, ethyl acetate,
methyl acetate, ethyl lactate, butanol, isopropanol or
ethanol, respectively) was done under conventionally
heated reflux. Twenty-five grams of blackcurrant bud
were precisely weighed and placed in a flask containing
100 mL of solvent. The buds were submerged and
extracted at the boiling point of each solvent for two hours.
The sample was filtered, then the oil in the flask was
desolventised in the rotary vacuum evaporator and finally
stored at 4 8C until used. All experiments were carried out
in triplicates