For Cu contaminated soils, the total variation explained by temperature and exposure time together was 63.4%, temperature and sampling times were responsible for 2.5 and 47.1%, respectively. Monte Carlo permutation test indicated a significant effect for all temperatures at 28 and 61 days for both control and Cu treatments when compared to 10◦C. Control soil SMS showed the highest significance over 84 days for the highest temperature and the only situation in which no significant difference occurred was at 19◦C. For the Cu contaminated soil the same results were observed until 61 days. After 84 days both 14◦C and 23◦C showed significant difference when compared to 10 ◦C and the highest significance was still seen for the highest temperature tested (Table 3)