The worldwide application of AI was rapidly adopted due to the ability to store semen for more than 10 days at 15–17◦C,which enables the trade both nationally and internationally(Gerrits et al., 2005; Guérin and Pozzi, 2005; Rodríguez Giland Estrada, 2013). However, it is well known that the process of semen collection in farm animals is a procedure that is not sterile due to multiple sources of contamination during the collection and manipulation processes (Bussalleuand Torner, 2013). These sources of contamination are clas-sified into those from animal and non-animal origin, suchas feces, preputial cavity fluids, water or ventilation system