Small pieces of muscle tissue were dissected out and fixed in Bouin's solution for routine microtome and 5 mm thick paraffin sections were stained by Masson's Trichrome Staining Protocol for muscle and collagen (Bancroft & Stevens, 1982). The sections were mounted with DPX and observed under light microscope with 400 magnification. Muscle fibres and collagen were stained red and green respectively.