Indeed, several questions remain unanswered [27,28]: Is the volatile oil from B. balsamifera safe for
the liver after transdermal absorption? If it is, what is the maximum effective dose without significant
toxicity and what is the role played by predisposing factors? If not, what is the adverse effect on liver
and degree of hepatotoxicity? With these queries in mind, we initiated screening studies of different
BB oil concentrations in rats to determine if any deleterious effects could be observed. Cutaneous
wound is a frequent condition in which BB oil is used. To assess the influence of predisposing factors,
we attempted to study the effect of BB oil under cutaneous wound conditions.