Soon after incubation, cells in cultures using L-15 (BM I) began to migrate from the explants and found attached with short pseudopodia developing a small network. No pseudopodial network was observed in cultures supplemented with GE whereas in ME a pseudopodial network of 100 μm width was recorded. A comparatively larger pseudopodial network of 150 μm could be found in WBE. The cell yield was at maximum in cultures using medium incorporated with ME and minimum in culture using BM 1 without any extract (Table 2). Significant difference in cell yield was observed between all the treatments, P = 0.002 (Table 3). Significant differences were not observed between treatments with BM I with GE and between media with ME and WBE. Maximum significance was observed between treatments using BM I and BM I with ME and BM I with GE and BM I with ME. Significant differences were also observed between treatments of BM I and BM I incorporated with WBE and between media incorporated with GE and WBE.