(Clontech vector containing the EGFP gene)
such that the ORF4a/gp33 fragment was adjacent to and in frame with the EGFP
gene. The ORF4a/gp33/EGFP portion of the plasmids was sequenced using
primers EGFP 530 and EGFP 766 (Table 1) and the Taq dye terminator
procedure according to the manufacturer’s protocol (Taq DyeDeoxy Terminator
Cycle Sequencing kit; Applied Biosystems)