Fig. 1. Amplification of 261 bp fragment using primers 261F and 261R on ten geographic isolates of MBV positive tissues. Lane M: 1 kb
molecular weight ladder Lanes 1–10: DNA extracted from MBV positive tissues. Lanes 1–4: Malaysia isolates (02MA1, 02MA2, 02MA3,
03MA, respectively). Lanes 5–7: Hawaii isolates (04HI1, 04HI2, 04HI3, respectively, these samples were introduced from the Philippines).
Lanes 8 and 10: Thailand isolates (01TH and 92TH, respectively). Lane 9: Taiwan isolate (91TW). Lane 11: SPF shrimp DNA. Lane 12: notemplate
(NT) control. The data is representative of three independent experiments.