Currently, the maximum possible consumption of isoflavone for an adult, at which estrogen-like effects can be expected, is considered to be approximately 40 mg/d. It has also been reported that isoflavone with the aglycon form has a higher systemic absorptivity than that with the glucoside form (10). From the comparison of aglycon content, approximately 250 g of the soybean tempeh should be consumed per day, whereas one-eighth or less of this amount of the new isoflavone-enriched tempeh is sufficient. Since glycitein content among the three types of aglycon is low in the soybean tempeh and genistein content is low in the soybean germ (11), the final proportions of the three types of aglycon become almost equal in the isoflavone-enriched tempeh made by adding the soybean germ. The taste of the isoflavone-enriched tempeh was not significantly different from that of the traditional soybean tempeh. Furthermore, since the defatted-soybean germ and cotyledon are granular, the isoflavone-enriched tempeh made of these materials may be applicable as a functional food for the elderly to suppress the onset of arteriosclerosis and alleviate osteoporosis symptoms which occur after menopause