Sample preparation (extraction and purification) was performed
in triplicate (n = 3) and the analyses of samples by HPLC
were carried out in duplicate (n = 2) so as to provide 6 data from
each truffle. The results expressed as mean values standard
deviation (SD). Analysis of variance was performed by ANOVA
procedures (SPSS 15.0), p < 0.05 was considered to be statistically
significant.