(40,6-diamidino-2-phenylindole)
for 15 min after hybridization. Fluorescent samples were examined
by a laser scanning confocal microscope (FV1000, Olympus, Japan).
The software ImageJ (http://rsbweb.nih.gov/ij/) was used for FISH
quantification and the proportions of AOB and NOB were determined
as the average areas to that of the total cells (at least 10
views of each sample).