PCR results were generated using the 5′-nuclease assay (TaqMan), if the probe is commercially available, and the ABI 7900HT Sequence Detection System (Applied Biosystems, Forster City, CA).
However, these findings of increased glycine concentration indicate a possible role played by glycine in the pathogenesis of HD and also suggest that glycine binding site of NMDA receptor could be an attractive target for HD drug development.