High Performance Liquid Chromatography (HPLC)
Polyphenol-containing extracts of C. officinalis flowers
were separated on a C18 (150 mm × 4.6 mm, 3 μm) column,
with a mobile phase consisting of three different
solvents, using an Agilent 1100 HPLC apparatus. The
flow rate was 0.7 mL/min and the injection volume was
20 μL. For the first separation system (ternary solvent),
which was used for the separation of flavanols and phenolic
acids, the initial mobile phase consisted of 2%