For determination of hepatic triglycerides in mice from Expt. 2, liver
tissue (50–100 mg) was homogenized in 2 mL isopropanol with a
Polytron disrupter. The homogenate was centrifuged at 2000 3 g;
10 min, and the supernatant was collected. Triglycerides in the supernatant
were measured with a Triglyceride H kit (Wako Diagnostics).