Extraction of cellular juice from fruit
The dried mature fruits of D. microcarpum were thoroughly washed under running tap water, dried in oven at 40ºC for 4 h, corticated manually to get rid of the inside hard seeds. The obtained dried pulp was ground to pass through 0.5 mm sieve by a grinding mill (Petra electric, Burgau, Germany) and then extracted by water at room temperature, filtered using normal filter.