To optimize extraction of aflatoxins from egg we weighed 1 g of the homogenized sample and mixed it with 5 ml of various extraction media: 1) water to which 0.05% Tween 20 had been added (0.05% Tween 20); 2) water: methanol 30:70 (methanol 70%); 3) water: methanol 70:30, in which 0.3 M NaCl had been added to water (methanol 70%/0.3 M NaCl); and 4) water: methanol 70:30 followed by the addition of hexane to remove fatty components.