The samplewas heated for 10min in boiling water to
gelatinize the starch. After cooling the gelatinized sample to room temperature,
the total volume was increased to 1 mL by adding distilled
water. Starch solution (500 μL) was mixed with 100 μL of 1 M acetic
acid. Then, 200 μL of iodine solution (0.2 g of iodine and 2.0 g of potassium
iodide in 100 mL of aqueous solution) was added