The dried aerial portion (1.8 kg) of J. betonica was extracted with hot EtOH-H2O (95:5,v/v) under reflux 4 times (each 8 l, 3 h, 70 C). The EtOH extract was concentrated to dryness (256.6 g) and partitioned between Et2O and H2O, with the aqueous soluble applied to column of highly porous copolymer resin of styrene divinylbenzene, and eluted with H2O, MeOH–(3:2), MeOH and Me2CO, successively.