A. suspensa were obtained from a laboratory colony maintained
at the USDA-ARS, Subtropical Horticulture Research Station
in Miami, FL. All flies were of known age and reared under the
following conditions: 25±2 ◦C, 75±5% RH, and a 12:12-h (L:D)
photoperiod (Kendra et al., 2006). Adults of mixed-sex (∼1:1 sex
ratio) were housed in screen rearing cages (30cm×30cm×30 cm)
and provisioned with water (released from agar blocks) and food
(refined cane sugar and yeast hydrolysate, 4:1 mixture) ad libitum
prior to collection for fruit infestation. Approximately 3500
mature (10–12 d old, presumed mated) females were collected by
aspiration from the rearing cages and placed in each of two infestation
cages (94cm×51cm×51 cm) constructed from PVC frames
covered with mesh pollination bags (Delstar Technologies, Middletown,
DE). Each cage contained 50 ripe Florida-grown grapefruit
(Citrus×paradisi cv. Marsh Red, obtained from a local natural foods
market) arranged in a single layer. Oviposition was allowed for 24 h,
and then the fruit was removed and rinsed with distilled water
to remove the fly excreta (and any potential volatiles it may have
liberated).