Multiple sequence alignments of pullulanases. The numbering refers to the amino acid position inB. acidopullulyticus. The bacterial species used for alignment
areAnaerobranca gottschalkii, Caldicellulosiruptor saccharolyticus, Fervidobacterium pennivorans, Geobacillus thermoleovorans, and Thermotoga maritime. The sequences were
aligned using ClustalW2