Batch fermentations were performed using a modified Lin’s medium, which was optimized during our experiment and gave the highest pigment production in the submerged flask culture experiment. Optimum agitation speed was determined to be 700 rpm in batch fermentation (data not shown). In submerged mold culture, mycelia may grow into the homogeneous and filamentous suspensions (so called pulp type growth), the globeose colonies (so called pellet type growth), or the intermediate forms between pulp and pellet type [21]. It was reported that cultivation at optimal agitation
speed produces mycelial forms of intermediate type and provides an effective means of increasing the pigment
yield [22]. At a speed of 700 rpm, the similar results were found that cells showed the intermediate forms between the pulp and pellet type, and pigment production was good. At a agitation speed of 900 rpm, pellet type mycelia predominated and pigment production was drastically decreased.
Batch fermentations were performed using a modified Lin’s medium, which was optimized during our experiment and gave the highest pigment production in the submerged flask culture experiment. Optimum agitation speed was determined to be 700 rpm in batch fermentation (data not shown). In submerged mold culture, mycelia may grow into the homogeneous and filamentous suspensions (so called pulp type growth), the globeose colonies (so called pellet type growth), or the intermediate forms between pulp and pellet type [21]. It was reported that cultivation at optimal agitationspeed produces mycelial forms of intermediate type and provides an effective means of increasing the pigmentyield [22]. At a speed of 700 rpm, the similar results were found that cells showed the intermediate forms between the pulp and pellet type, and pigment production was good. At a agitation speed of 900 rpm, pellet type mycelia predominated and pigment production was drastically decreased.
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