The objective of this study was to establish cell suspension culture and plant regeneration via
somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers were
used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were
used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five
semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant
regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in
the MS medium, with 11.4 μM indolacetic acid and 2.2 μM 6-benzylaminopurine. Regenerated plants showed
a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate
plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 μM
of IAA and 2.2 μM of BAP.
The objective of this study was to establish cell suspension culture and plant regeneration viasomatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male fl owers wereused as explant source for generating highly embryogenic cultures 45 days after inoculation, which wereused for establishment of cell suspension culture and multiplication of secondary somatic embryos. Fivesemisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plantregeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated inthe MS medium, with 11.4 μM indolacetic acid and 2.2 μM 6-benzylaminopurine. Regenerated plants showeda normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerateplants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 μMof IAA and 2.2 μM of BAP.
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