Fish Barcode of Life Initiative (FISH-BOL), as a campaign of the International Barcode of Life Project (iBOL), is to build up
a standardized database of reference sequences for all fishes. With regard to fishes, the target DNA segment of 652 base-pairs
near 50 end of the mitochondrial cytochrome oxidase subunit I gene is strongly proposed (Hebert et al., 2003a). These
sequences are derived from voucher specimens preserved in the museums all around the world. Storage information
(taxonomic identification, catalogue number and institution storing) and collection data (collector, collection date and
location with longitude-latitude coordinates) are required to inject specimen records when creating projects for them. In
addition, DNA barcodes (sequences of at least 500 bps and trace files) need to be uploaded to projects in FISH-BOL after
finishing PCR amplification and sequencing. Thus, any problem concerning morphological identification can be solved by
searching the relative data in BOLD or sending enquiries for checking voucher specimens preserved in natural history
collections. Assignment of specimens to known species can be achieved through the comparison of genetic similarity with thesequence database. FISH-BOL provides a platform for ichthyologists to collaborate more closely, but also greatly accelerate
species identification and discovery (Swartz et al., 2008).
In this study, a data set composed of 229 sequences of mitochondrial cytochrome oxidase subunit I gene (COI) from 158
species, some of which are represented by multiple sequences,was employed to elucidate the rationale of DNA barcoding and
test its accuracy in species identification.
Fish Barcode of Life Initiative (FISH-BOL), as a campaign of the International Barcode of Life Project (iBOL), is to build upa standardized database of reference sequences for all fishes. With regard to fishes, the target DNA segment of 652 base-pairsnear 50 end of the mitochondrial cytochrome oxidase subunit I gene is strongly proposed (Hebert et al., 2003a). Thesesequences are derived from voucher specimens preserved in the museums all around the world. Storage information(taxonomic identification, catalogue number and institution storing) and collection data (collector, collection date andlocation with longitude-latitude coordinates) are required to inject specimen records when creating projects for them. Inaddition, DNA barcodes (sequences of at least 500 bps and trace files) need to be uploaded to projects in FISH-BOL afterfinishing PCR amplification and sequencing. Thus, any problem concerning morphological identification can be solved bysearching the relative data in BOLD or sending enquiries for checking voucher specimens preserved in natural historycollections. Assignment of specimens to known species can be achieved through the comparison of genetic similarity with thesequence database. FISH-BOL provides a platform for ichthyologists to collaborate more closely, but also greatly acceleratespecies identification and discovery (Swartz et al., 2008).In this study, a data set composed of 229 sequences of mitochondrial cytochrome oxidase subunit I gene (COI) from 158species, some of which are represented by multiple sequences,was employed to elucidate the rationale of DNA barcoding andtest its accuracy in species identification.
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