MS medium was augmented with plant growth regulators viz., Indole-3-acetic acid (IAA), -naphthalene acetic acid (NAA) and Benzyl adenine (BA) at different concentrations were used in this study. Sucrose was used as the carbon source at the rate of 30 gl-1 and 0.8% agar used in all experiments. The pH of the medium was adjusted to 5.8 and sterilized by autoclaving at 121oC for 20 min at 15lbs pressure. The cultures were incubated at 25±1oC and were given a photoperiod of 16 h with a light intensity of 3000 lux.